Exonuclease I (E.coli) from New England Biolabs

Product Specs
ItemExonuclease I (E.coli)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0293S
Quantity3000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
Amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Single-cell bisulfite-free 5mC and 5hmC sequencing with high sensitivity and scalabilityProceedings of the National Academy of SciencesDecember 5, 2023Yunlong Cao, Yali Bai, Tianjiao Yuan, Liyang Song, Yu Fan, Liuhao Ren, Weiliang Song, Jiahui Peng, Ran An, Qingqing Gu, et al.and 65 °C for 5 min. Excessive primers were digested with the addition of 0.5 μL Exonuclease I (NEB M0293S), and incubation at 37 °C for 30 min and 72 °C for 20 min. Library construction was completed by adding

(2) Different PfEMP1-expressing Plasmodium falciparum variants induce divergent endothelial transcriptional responses during co-culturePLOS ONENovember 30, 2023Basim Othman, Leo Zeef, Tadge Szestak, Zineb Rchiad, Janet Storm, Caroline Askonas, Rohit Satyam, Aymen Madkhali, Michael Haley, Simon Wagstaff, et al.(100–6472 B1, PN 100–5875 C1) and reagents (Fluidigm,100–6297; Fluidigm, 100–5580; New England Biolabs, M0293S; TEKnova, 10221). Real-time PCR data was collected using the Biomark HD system with an 96.96 IFC chip

(3) Detection of isoforms and genomic alterations by high-throughput full-length single-cell RNA sequencing in ovarian cancerNature CommunicationsNovember 27, 2023Arthur Dondi, Ulrike Lischetti, Francis Jacob, Franziska Singer, Nico Borgsmüller, Ricardo Coelho, Rudolf Aebersold, Melike Ak, Faisal S. Al-Quaddoomi, Silvana I. Albert, et al.addition of 5 µL of NEBbuffer1 (NEB, cat. no. B7001S) and 1 µL of each Exonuclease I (NEB, cat. no. M0293S) and Exonuclease III (NEB, cat. no. M0206S) bringing the total volume to 50 µL per reaction. Enzyme

(4) Connecting Chromatin Structures to Gene Regulation Using Dynamic Polymer SimulationsbioRxivNovember 15, 2023Yi Fu, Finnegan Clark, Sofia Nomikou, Aristotelis Tsirigos, Timothee Lionnetincubated at 50 °C for 3 h. 11 uL Exonuclease I Buffer and 2 uL Exonuclease I (New England Biolabs; M0293S) was added following reverse transcription and incubated at 37 °C for 15 min. Then 12 uL 0.5 M EDTA

(5) Gain without pain: adaptation and increased virulence of Zika virus in vertebrate host without fitness cost in mosquito vectorJournal of VirologyOctober 31, 2023Anna S. Jaeger, Jeffrey Marano, Kasen K. Riemersma, David Castaneda, Elise M. Pritchard, Julia C. Pritchard, Ellie K. Bohm, John J. Baczenas, Shelby L. O'Connor, James Weger-Lucarelli, et al.assembly products were digested with DpnI (R0176S), lambda exonuclease (M0262S), and exonuclease I (M0293S), and amplified using SuperPhi RCA Premix Kit with Random Primers (Evomic Science catalog number PM100)

(6) Proton Microbeam Targeted Irradiation of the Gonad Primordium Region Induces Developmental Alterations Associated with Heat Shock Responses and Cuticle Defense in Caenorhabditis elegansBiology (Basel)October 27, 2023Pierre Beaudier, Guillaume Devès, Laurent Plawinski, Denis Dupuy, Philippe Barberet, Hervé Seznecamplify cDNAs up to ~5 kb in length.) 1 µL Exonuclease 1 (New England Biolabs, Evry, France, cat. no. M0293S) was finally added in each PCR tube for incubation at +37 °C, followed by enzyme inactivation at +80

(7) The admixed brushtail possum genome reveals invasion history in New Zealand and novel imprinted genesNature CommunicationsOctober 17, 2023Donna M. Bond, Oscar Ortega-Recalde, Melanie K. Laird, Takashi Hayakawa, Kyle S. Richardson, Finlay.C. B. Reese, Bruce Kyle, Brooke E. McIsaac-Williams, Bruce C. Robertson, Yolanda van Heezik, et al.(BioP5N7) with intermediate heat denaturation of the DNA fragments89. Exonuclease I (New England Biolabs, M0293S) was added to the first strand synthesis product to remove any remaining adapter primer, and the product

(8) Multi-faceted attributes of salivary cell-free DNA as liquid biopsy biomarkers for gastric cancer detectionBiomarker ResearchOctober 10, 2023Neeti Swarup, Jordan Cheng, Irene Choi, You Jeong Heo, Misagh Kordi, Mohammad Aziz, Akanksha Arora, Feng Li, David Chia, Fang Wei, et al.8). ssDNA-specific digestion Twenty microliters of cfDNA was combined with 3 µL of Exonuclease 1 (NEB, M0293S), 3 µL of 10x Exo 1 Buffer, and 4 µL of ddH2O, incubated for 30 min at 37 °C and heat inactivated for

(9) NPD1 Plus RvD1 Mediated Ischemic Stroke Penumbra Protection Increases Expression of Pro-homeostatic Microglial and Astrocyte GenesCellular and Molecular NeurobiologyOctober 1, 2023Madigan M. Reid, Marie-Audrey I. Kautzmann, Gethein Andrew, Andre Obenaus, Pranab K. Mukherjee, Larissa Khoutorova, Jeff X. Ji, Cassia R. Roque, Reinaldo B. Oria, Bola F. Habeb, et al.unincorporated primers after the STA reaction, samples were treated with exonuclease I (Escherichia coli; Cat. #M0293S, New England Biolabs). To the STA samples, 2 µL of the exonuclease reaction mixture was added, and digestion

(10) Integrated inflammatory signaling landscape response after delivering Elovanoid free-fatty-acid precursors leading to experimental stroke neuroprotectionScientific ReportsSeptember 22, 2023Madigan M. Reid, Ludmila Belayev, Larissa Khoutorova, Pranab K. Mukherjee, Andre Obenaus, Kierany Shelvin, Stacey Knowles, Sung-Ha Hong, Nicolas G. Bazaneliminate unincorporated primers post-STA, samples were treated with exonuclease I (E. coli; Cat. #M0293S, New England Biolabs). A 2 µL exonuclease reaction mixture was added to the STA samples. Digestion with

(11) Cfdp1 Is Essential for Cardiac Development and FunctionCellsAugust 3, 2023Panagiota Giardoglou, Panos Deloukas, George Dedoussis, Dimitris Beisprepared for Sanger sequence after product cleaning using ExoSAP (New England Biolabs, Ipswich, MA, USA #M0293S, #M0371S). Alternatively, amplicons were visualized on a 2% agarose gel after diagnostic digestion to

(12) Key role of phosphorylation sites in ATPase domain and Linker region of MLH1 for DNA binding and functionality of MutLαSci RepAugust 2, 2023May-Britt Firnau, Guido Plotz, Stefan Zeuzem, Angela Briegerwas transferred to a new test tube. 10 µl of this supernatant were digested with 5U of exonuclease I (M0293S (20U/µl), New England Biolabs GmbH, Frankfurt, Germany) and incubated for 1 h at 37 °C to remove residual

(13) Scalable co-sequencing of RNA and DNA from individual nucleibioRxivJuly 11, 2023Timothy R. Olsen, Pranay Talla, Julia Furnari, Jeffrey N. Bruce, Peter Canoll, Shan Zha, Peter A. Simsfor 2 minutes; 75 °C for 10 minutes; 4 °C hold). Then, 1.25 µL of exonuclease I (New England Biolabs #M0293S) and 12.08 µL of H2O were added to the RT product and incubated on a thermocycler (37 °C for 30 minutes,

(14) Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the Bdelloid rotifer Adineta vagaJournal of Biological ChemistryJuly 5, 2023Jujun Zhou, John R. Horton, Gundeep Kaur, Qin Chen, Xuwen Li, Fabian Mendoza, Tao Wu, Robert M. Blumenthal, Xing Zhang, Xiaodong Chengstreptavidin beads. The supernatant was transferred to a new tube and treated with 1 μl Exonuclease I (NEB, M0293S) at 37 °C for 15 min and 70 °C for 10 min to remove excessive R2-22nt primer. Exonuclease I–treated

(15) Missense mutations in CRX homeodomain cause dominant retinopathies through two distinct mechanismsbioRxivJune 20, 2023Yiqiao Zheng, Chi Sun, Xiaodong Zhang, Philip A. Ruzycki, Shiming ChenLadder,LI-COR,928–60000 Dithiothreitol (DTT),Bio-Rad Laboratories,1610611 Exonuclease I (E. coli),New England Biolabs,M0293S Gibco™ Dulbecco’s Modified Eagle Medium,ThermoFisher Scientific,11965084 Gibco™ Fetal Bovine Serum,ThermoFisher Scientific

(16) Osteoarthritic chondrocytes undergo a glycolysis-related metabolic switch upon exposure to IL-1b or TNFCell Communication and SignalingJune 14, 2023Anais Defois, Nina Bon, Alexandre Charpentier, Melina Georget, Nicolas Gaigeard, Frederic Blanchard, Antoine Hamel, Denis Waast, Jean Armengaud, Ophelie Renoult, et al.samples were then pooled and purified using a Zymo kit. cDNAs were treated with exonuclease I (NEB, M0293S) before being amplified (12 cycles) and fragmented using a transposon-fragmentation approach that enriched

(17) An ERK1/2-driven RNA-binding switch in nucleolin drives ribosome biogenesis and pancreatic tumorigenesis downstream of RAS oncogeneThe EMBO journalJune 1, 2023Azman MS, Alard EL, Dodel M, Capraro F, Faraway R, Dermit M, Fan W, Chakraborty A, Ule J, Mardakheh FK.tube,VWR,713‐2536 Glycoblue,Ambion,9510 Superscript IV reverse transcriptase kit,Thermo Fisher,18090010 Exonuclease I,NEB,M0293S Agencourt AMPure XP beads,Beckman Coulter,A63880 CircLigase II ssDNA ligase kit,Epicentre,CL9021K Phusion

(18) Control-independent mosaic single nucleotide variant detection with DeepMosaicNature BiotechnologyJune 1, 2023Xiaoxu Yang, Xin Xu, Martin W. Breuss, Danny Antaki, Laurel L. Ball, Changuk Chung, Jiawei Shen, Chen Li, Renee D. George, Yifan Wang, et al.M7832) on sperm, blood, and an unrelated control. Amplicons were enzymatically cleaned with ExoI (NEB, M0293S) and SAP (NEB, M0371S) treatment. Following normalization with the Qubit HS Kit (ThermoFisher Scientific,

(19) Regulatory architecture of housekeeping genes is driven by promoter assembliesCell reportsMay 30, 2023Dejosez M, Dall'Agnese A, Ramamoorthy M, Platt J, Yin X, Hogan M, Brosh R, Weintraub AS, Hnisz D, Abraham BJ, et al.volume of 1.5 mL. Unligated DNA was digested with exonuclease (NEB, M0262S) and lambda nuclease (NEB, M0293S). The ligated DNA was eluted in elution buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS), and then

(20) DNA supercoiling restricts the transcriptional bursting of neighboring eukaryotic genesMolecular CellMay 18, 2023Heta P. Patel, Stefano Coppola, Wim Pomp, Umberto Aiello, Ineke Brouwer, Domenico Libri, Tineke L. Lenstra03115887001 SuperScript IV Reverse Transcriptase,Thermo Fisher Scientific,Cat# 18090050 Exonuclease I,NEB,Cat# M0293S RNase H,NEB,Cat# M0297S LA Taq,Takara,Cat# RR002M Zymolase 100T,US biological,Cat# Z1004.250 Micrococcal

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